persmedРоссийский журнал персонализированной медициныRussian Journal for Personalized Medicine2782-38062782-3814ФОНД АЛМАЗОВА10.18705/2782-3806-2024-4-3-268-275IJWRSNpersmed-271Research ArticleКЛЕТОЧНАЯ БИОЛОГИЯ, ЦИТОЛОГИЯ, ГИСТОЛОГИЯАналитические подходы подготовки проб для нецелевого метаболомного анализа адгезивных клеточных культурUntargeted metabolomic analysis of adherent cell cultures: general recommendations for sample preparationКессенихЕ. Д.KessenikhE. D.

Кессених Елизавета Дмитриевна, научный сотрудник НИЛ метаболомного и метаболического профилирования НИЦ неизвестных, редких и генетически обусловленных заболеваний НЦМУ «Центр персонализированной медицины»

ул. Аккуратова, д. 2, Санкт-Петербург, 197341

Kessenikh Elizaveta D., researcher, Laboratory of metabolomic and metabolic profiling, Research Centre of unknown, rare and genetically determined diseases, World-Class Research Centre for Personalized Medicine

Akkuratova str., 2, Saint Petersburg, 197314

kessenikh_ed@almazovcentre.ruМигуноваМ. А.MigunovaM. A.

Мигунова Маргарита Александровна, лаборант-исследователь НИЛ метаболомного и метаболического профилирования НИЦ неизвестных, редких и генетически обусловленных заболеваний НЦМУ «Центр персонализированной медицины»

ул. Аккуратова, д. 2, Санкт-Петербург, 197341

Migunova Margarita A., research assistant, Laboratory of metabolomic and metabolic profiling, Research Centre of unknown, rare and genetically determined diseases, World-Class Research Centre for Personalized Medicine

Akkuratova str., 2, Saint Petersburg, 197314

КривошеинаМ. И.KrivosheinaM. I.

Кривошеина Мария Игоревна, лаборант-исследователь НИЛ метаболомного и метаболического профилирования НИЦ неизвестных, редких и генетически обусловленных заболеваний НЦМУ «Центр персонализированной медицины»

ул. Аккуратова, д. 2, Санкт-Петербург, 197341

Krivosheina Maria I., research assistant, Laboratory of metabolomic and metabolic profiling, Research Centre of unknown, rare and genetically determined diseases, World-Class Research Centre for Personalized Medicine

Akkuratova str., 2, Saint Petersburg, 197314

МурашкоЕ. А.MurashkoE. A.

Мурашко Екатерина Александровна, к.х.н., заведующий НИЛ метаболомного и метаболического профилирования НИЦ неизвестных, редких и генетически обусловленных заболеваний НЦМУ «Центр персонализированной медицины»; ассистент кафедры математики и естественнонаучных дисциплин Института медицинского образования 

ул. Аккуратова, д. 2, Санкт-Петербург, 197341

Murashko Ekaterina A., Ph.D, head of laboratory, Laboatory of metabolomic and metabolic profiling, Research Centre of unknown, rare and genetically determined diseases, World-Class Research Centre for Personalized Medicine

Akkuratova str., 2, Saint Petersburg, 197314

Федеральное государственное бюджетное учреждение «Национальный медицинский исследовательский центр имени В. А. Алмазова» Министерства здравоохранения Российской ФедерацииРоссияAlmazov National Medical Research CentreRussian Federation20240908202443268275Copyright © Кессених Е.Д., Мигунова М.А., Кривошеина М.И., Мурашко Е.А., 20242024Кессених Е.Д., Мигунова М.А., Кривошеина М.И., Мурашко Е.А.Kessenikh E.D., Migunova M.A., Krivosheina M.I., Murashko E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://persmed.elpub.ru/jour/article/view/271

Метаболомика представляет собой количественный и качественный анализ максимально полного набора метаболитов, присутствующих в клетках, биологических жидкостях и тканях, с использованием хемометрического и статистического анализа различий между метаболомами исследуемых групп. Надежность полученных аналитических данных и, как следствие, достоверная биологическая интерпретация результатов метаболомного исследования обусловлены выбором надлежащих процедур преаналитического этапа.

В обзоре представлены общие рекомендации по планированию и организации нецелевого метаболомного исследования с использованием адгезивных клеточных культур. Рассмотрены основные стратегии оптимизации процедуры подготовки проб к анализу и выбора условий культивирования клеточных культур, отбора проб, снижения метаболической активности и экстракции метаболитов.

Metabolomics is a comprehensive quantitative and qualitative analysis of metabolites in biological specimens (cells, biological fluids and tissues). It includes chemometric and statistical analysis of metabolomic data to assess group-wise differences. The reliability of the analytical data and the biological meaningful results of the metabolomics study are determined by the selection of appropriate procedures of sample preparation.

The review outlines general recommendations for planning and organizing untargeted metabolomics studies of adherent cell cultures. The main strategies and procedures for optimization of sample preparation and selection of culture conditions, sampling, metabolism quenching and metabolite extraction are considered.

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The authors declare that there are no conflicts of interest present.