Preview

Russian Journal for Personalized Medicine

Advanced search

Pseudothrombocytopenia — problems and ways to solve diagnostic problems

https://doi.org/10.18705/2782-3806-2023-3-4-38-43

Abstract

EDTA-dependent pseudothrombocytopenia is the laboratory phenomenon of a spurious low platelet count which appears in vitro while using EDTA as anticoagulant. The problem of EDTA-dependent pseudothrombocytopenia has significant relevance due to the widespread using of automated hematology analyzers which EDTA stabilized blood is most recommended for. The mechanism of this phenomenon is based on the production of the specificantibodies causing the platelet aggregation in the presence of EDTA. The aim of this study was to evaluate MgSO4 as an in vitro anticoagulant for platelet count, MPV and correction WBC. Early determination of the patients with EDTA-dependent pseudothrombocytopenia is an important diagnostic criterion necessary to exclude blood diseases and prevent errors in treatment.

About the Authors

M. N. Zenina
North-Western State Medical University named after I. I. Mechnikov; Russian Research Institute of Hematology and Transfusiology of the Federal Medical and Biological Agency of Russia
Russian Federation

Zenina Marina N. - Head of the Department of Morphological Research of the Center for Laboratory Research of the Clinic

Kirochnaya str., 41, Saint Petersburg, 191015



M. A. Nuzhdina
Russian Research Institute of Hematology and Transfusiology of the Federal Medical and Biological Agency of Russia
Russian Federation

Nuzhdina Maria A. - Doctor of Clinical Laboratory Diagnostics, Department of Morphological Research, Center for Laboratory Research Clinic



N. Y. Chernysh
Russian Research Institute of Hematology and Transfusiology of the Federal Medical and Biological Agency of Russia; Almazov National Medical Research Centre
Russian Federation

Chernysh Natalia Y. - PhD, Associate Professor of the Department of Laboratory Medicine with a clinic ; Doctor of Clinical Laboratory Diagnostics, Department of Morphological Research, Center for Laboratory Research Clinic



V. A. Yudina
Russian Research Institute of Hematology and Transfusiology of the Federal Medical and Biological Agency of Russia; Almazov National Medical Research Centre
Russian Federation

Yudina Victoria A. - PhD, Head of the Center for Laboratory Research of the Clinic; Associate Professor of the Department of Laboratory Medicine with the Clinic



References

1. Lin J, Luo Y, Yao S, et al. Discovery and Correction of Spurious Low Platelet Counts due to EDTA-Dependent Pseudothrombocytopenia // Journal of Clinical Laboratory Analysis. 2015. Vol. 29. P. 419– 426.

2. Harrison P, Ault K, Chapman S, et al. An Interlaboratory Study of a Candidate Reference Method for Platelet Counting // Am J Clin Pathol. 2001. Vol.115. P. 448–459.

3. Луговская С.А., Почтарь М.Е., Долгов В.В. Гематологические анализаторы. Интерпретация анализа крови: Методические рекомендации. М.-Тверь: ООО «Издательствоо «Триада». 2007. 112 с.

4. Мининкова А.И. Аналитические возможности гематологических анализаторов в оценке тромбоцитов (обзор литературы)// Клиническая лабораторная диагностика. 2012. № 3. C. 27–34.

5. Соколова Н.А. Преимущества и недостатки различных методов подсчета тромбоцитов //Справочник заведующего КДЛ. 2020. № 6. С. 14–29.

6. Соснин Д.М. Роль микроскопических исследований при подсчете тромбоцитов // Справочник заведующего КДЛ. 2018. № 12. C. 24–32.

7. Sheu J, Hsiao G, Shen M, et al. Mechanisms involved in the antiplatelet activity of magnesium in human platelets// Br J Haematol. 2002. Vol 119. Р. 1033– 1041.

8. Папаян К.А., Цветкова Т.Г., Андреева Э.Ф. и др. Значение своевременной диагностики ЭДТА-ассоциированной псевдотромбоцитопении в клинической практике// Forcipe. 2018. Т. 1. № 1. С. 4–8


Review

For citations:


Zenina M.N., Nuzhdina M.A., Chernysh N.Y., Yudina V.A. Pseudothrombocytopenia — problems and ways to solve diagnostic problems. Russian Journal for Personalized Medicine. 2023;3(4):38-43. (In Russ.) https://doi.org/10.18705/2782-3806-2023-3-4-38-43

Views: 285


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2782-3806 (Print)
ISSN 2782-3814 (Online)