Comparative Analysis of CAR T Cells with Dual Specificity for CD19 and CD20
https://doi.org/10.18705/2782-3806-2024-4-5-413-420
EDN: EENWVA
Abstract
CAR T-cell therapy of patients with B-cell malignancies demonstrates high efficacy and an acceptable safety profile. However, some patients do not respond to treatment or quickly relapse. One of the reasons for an inadequate response to CAR T-cell therapy may be the emergence of cancer cells escape variants that do not express the epitope recognized by the CAR. Using CAR T-cells with dual specificity could help mitigate this issue. In this study, we developed CAR T-cells specific to human CD19 and CD20 by exploring four configurations of antigen-recognition domains: two in the biCAR format and two in the dualCAR format. Expression of two independent CARs (dualCAR) driven by a single promoter was found to be the most promising format.
Supporting agencies: The work was carried out with the financial support of the Ministry of Science and Higher Education of the Russian Federation (Agreement No. 075-15-2022301 dated 04/20/2022)
About the Authors
T. N. Belovezhets
Almazov National Medical Research Centre
Russian Federation
Russian Federation
Belovezhets Tatyana N. - scientist, genome editing group, World Class Research Centre for Personalized Medicine.
Saint Petersburg
A. A. Gorchakov
Almazov National Medical Research Centre
Russian Federation
Russian Federation
Gorchakov Andrey A. - Candidate of Biological Sciences, senior scientist, genome editing group, World Class Research Centre for Personalized Medicine.
Saint Petersburg
K. A. Samochernykh
Almazov National Medical Research Centre
Russian Federation
Russian Federation
Samochernykh Konstantin A. - Doctor of Medical Sciences, Professor of the Russian Academy of Sciences, Head.
Saint Petersburg
S. V. Kulemzin
Almazov National Medical Research Centre
Russian Federation
Russian Federation
Kulemzin Sergei V. - Candidate of Biological Sciences, senior scientist, genome editing group, World Class Research Centre for Personalized Medicine.
Akkuratova str., 2, Saint Petersburg, Russia, 197341
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Review
For citations:
Belovezhets T.N., Gorchakov A.A., Samochernykh K.A., Kulemzin S.V. Comparative Analysis of CAR T Cells with Dual Specificity for CD19 and CD20. Russian Journal for Personalized Medicine. 2024;4(5):413-420. (In Russ.) https://doi.org/10.18705/2782-3806-2024-4-5-413-420. EDN: EENWVA
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