Аналитические подходы подготовки проб для нецелевого метаболомного анализа адгезивных клеточных культур

Метаболомика представляет собой количественный и качественный анализ максимально полного набора метаболитов, присутствующих в клетках, биологических жидкостях и тканях, с использованием хемометрического и статистического анализа различий между метаболомами исследуемых групп. Надежность полученных аналитических данных и, как следствие, достоверная биологическая интерпретация результатов метаболомного исследования обусловлены выбором надлежащих процедур преаналитического этапа.

В обзоре представлены общие рекомендации по планированию и организации нецелевого метаболомного исследования с использованием адгезивных клеточных культур. Рассмотрены основные стратегии оптимизации процедуры подготовки проб к анализу и выбора условий культивирования клеточных культур, отбора проб, снижения метаболической активности и экстракции метаболитов.

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