FEATURES OF COLLECTING BIOLOGICAL MATERIAL IN THE STUDY OF PATHOLOGICAL CALCIFICATION OF THE HEART AND BLOOD VESSELS

Abstract

Calcification of the heart and blood vessels is a common pathological condition, leading to numerous complications. One of the most common forms of calcification is aortic valve calcification. To date, there is no drug therapy that can stop the progression of calcification, so the only radical method of treatment remains surgery. Fundamental molecular biological studies and, accordingly, a relevant in vitro model are necessary to search for an anticalcifying therapy. In the article, we present the organization of the work of a translational group, consisting of clinicians, surgeons, and molecular biologists, working with cellular models of calcification. As cell models, a description is given of obtaining cell lines of interstitial and endothelial cells of the aortic valve, as well as smooth muscle and endothelial cells of the aorta. The preparatory clinical and surgical stages of obtaining a biomaterial, and the laboratory stage of working with the material are described.