THROMBIN GENERATION TEST AS AN INTEGRAL ANALYSIS OF THE HEMOSTASIS SYSTEM: TECHNICAL CAPABILITIES AND APPLICATION IN LABORATORY PRACTICE
https://doi.org/10.18705/2782-3806-2022-2-3-119-128
Abstract
This review is devoted to one of the integral tests for assessing the hemostasis system — the thrombin generation test (TGT), its technical characteristics, problems in standardization and possible clinical use. Evaluation of thrombin generation (TG) is more sensitive to changes occurring in the hemostasis system, since it takes into account the effect of both procoagulant and anticoagulant factors in the process of TG. It is important to note that there are options for setting TGT in platelet-rich plasma or in whole blood, which brings the researcher closer to in vivo conditions. However, despite the obvious advantages of this analysis when compared with routine screening tests for assessing the hemostasis system, there are number of limitations, including the lack of standardization, which does not currently allow the introduction of TGT into clinical practice. This review discusses the technical characteristics of TGT and variants of reagent kits depending on the clinical task, and provides the results of recent studies in the field of clinical use of TGT, demonstrating the prospects of GT analysis for assessing the risk of both hemorrhagic complications and thrombotic events.
About the Authors
O. S. Melnichnikova
Almazov National Medical Research Centre, World-Class Research Centre for Personalized Medicine
Russian Federation
Russian Federation
Melnichnikova Olga S., PhD, Senior Researcher, Research Group of Cardio-Oncology
Akkuratova str., 2, Saint Petersburg, 197341
Y. I. Zhilenkova
Almazov National Medical Research Centre, World-Class Research Centre for Personalized Medicine
Russian Federation
Russian Federation
Zhilenkova Yulia I., PhD., Assistant Professor, Laboratory Medicine and Genetics Department
Akkuratova str., 2, Saint Petersburg, 197341
E. A. Zolotova
Almazov National Medical Research Centre, World-Class Research Centre for Personalized Medicine
Russian Federation
Russian Federation
Zolotova Ekaterina A., Junior Researcher, Research Group of Cardio-Oncology
Akkuratova str., 2, Saint Petersburg, 197341
K. A. Pishchulov
Almazov National Medical Research Centre, World-Class Research Centre for Personalized Medicine
Russian Federation
Russian Federation
Pishchulov Konstantin A., Junior Researcher, Research Group of Cardio-Oncology
Akkuratova str., 2, Saint Petersburg, 197341
O. V. Sirotkina
Almazov National Medical Research Centre, World-Class Research Centre for Personalized Medicine
Russian Federation
Russian Federation
Sirotkina Olga V., D.Sc., Chief Researcher, Research Group of Cardio-oncology, professor of Department of Laboratory Medicine and Genetics
Akkuratova str., 2, Saint Petersburg, 197341
M. A. Simakova
Almazov National Medical Research Centre, World-Class Research Centre for Personalized Medicine
Russian Federation
Russian Federation
Simakova Maria A., PhD., Head, Senior Researcher, Research Group of Cardio-Oncology
Akkuratova str., 2, Saint Petersburg, 197341
T. V. Vavilova
Almazov National Medical Research Centre, World-Class Research Centre for Personalized Medicine
Russian Federation
Russian Federation
Vavilova Tatiana V., MD, D.Sc., Head, Laboratory Medicine and Genetics Department
Akkuratova str., 2, Saint Petersburg, 197341
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Review
For citations:
Melnichnikova O.S., Zhilenkova Y.I., Zolotova E.A., Pishchulov K.A., Sirotkina O.V., Simakova M.A., Vavilova T.V. THROMBIN GENERATION TEST AS AN INTEGRAL ANALYSIS OF THE HEMOSTASIS SYSTEM: TECHNICAL CAPABILITIES AND APPLICATION IN LABORATORY PRACTICE. Russian Journal for Personalized Medicine. 2022;2(3):119-128. (In Russ.) https://doi.org/10.18705/2782-3806-2022-2-3-119-128
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